Abstract:
The Effect of Histone Deacetylase Inhibitor Combined with Chemotherapy on Breast CancerCells in Nude MiceJianyun NIE, Yangping CHEN, Yunchao HUANGCorresponding author: Yunchao HUANG, E-mail: bluesky9@163.comDepartment of Breast Cancer, The Third Affiliated Hospital of Kunming Medical College, Kunming 650118, ChinaThis work was supported by Project of Science and Technology of Yunnan Province ( No.2007C0026R ) and Scientific ResearchFoundation for the Returned Overseas Chinese Scholars, State Education Ministry ( No. 2007 -170 and 2009 -416 )Abstract Objective: To study the effect of histone deacetylase inhibitor SAHA (N-Hydroxy-N- phenyloctanediamide)combined with chemotherapy on breast cancer cells in nude mice. Methods: Breast cancer cell suspension was subcutaneously injectedinto 80 nude mice to establish breast cancer model. When the transplanted tumor was about 10mm in length, 60 mice with tumor insimilar size were selected and divided into six groups, with 10 in each group. The six groups were the control group, SAHA group,taxol group, adriamycin group, taxol plus SAHA group, and adriamycin plus SAHA group. Data including blood cell count, serum levelof cholesterol, liver function ( serum glutamic-pyruvic transaminase and bilirubin ), kidney function ( serum urea nitrogen andcreatinine ), body weight, wet weight of tumor, inhibitory rate and tumor volume were collected. SPSS 12.0 software was used forstatistical analysis. Results: Compared with the control group, the treatment groups had higher inhibitory rate and lower tumor volume( P < 0.05). The taxol plus SAHA group showed superiority in inhibiting tumor growth ( P < 0.05). Compared with the control group,the groups treated with taxol or adriamycin had lower body weight, lower level of white blood cell count, lower level of serumglutamic-pyruvic transaminase and higher level of bilirubin ( P < 0.05 ). No differences were found in the above indices betweenchemotherapy groups and SAHA plus chemotherapy groups ( P > 0.05 ). Conclusion: SAHA combined with chemotherapy (taxol oradriamycin ) has synergistic inhibitory effect on the growth of breast cancer in nude mice, without increasing side effects, suggesting itspossible application in breast cancer treatment in the future.Keywords Breast neoplasm; Histone deacetylase inhibitor; Taxol; Adriamycin; Animal model